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Lack of CFAP54 causes primary ciliary dyskinesia in a mouse model and human patients

Frontiers of Medicine doi: 10.1007/s11684-023-0997-7

Abstract: A Cfap54 knockout mouse model was then reported to have PCD-relevant phenotypes.In addition, a CFAP54 in-frame variant knock-in mouse model was established, which recapitulated

Keywords： primary ciliary dyskinesia CFAP54 cilia

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Recombinant protein diannexin prevents preeclampsia-like symptoms in a pregnant mouse model via reducing

Frontiers of Medicine 2022, Volume 16, Issue 6, Pages 919-931 doi: 10.1007/s11684-021-0918-6

Abstract: Preeclampsia (PE) is characterized by placenta-mediated pregnancy complication. The only effective treatment for PE is the delivery of the placenta. However, this treatment may cause preterm birth and neonatal death. Therefore, preventing PE is needed. The mechanism of PE involves abnormal placentation, which leads to the release of anti-angiogenic and inflammatory mediators into maternal circulation. These mediators contribute to systemic vascular dysfunction, inflammatory responses, and excessive thrombin generation. Microparticles (MPs) are reportedly involved in PE by promoting the thromboinflammatory response. This study describes a strategy to prevent PE by reducing MP release using the recombinant protein, diannexin. Results showed that the patients with PE had elevated MP number and procoagulant activity and increased NLRP3 inflammasome activation. Additionally, diannexin remarkably reduced the release of MPs from activated cells by binding to phosphatidylserine exposed on the surface of activated cells. Moreover, in vivo results showed that diannexin could prevent PE-like symptoms by decreasing MPs and NLRP3 inflammasome activation in pregnant mice. Furthermore, diannexin effectively inhibited trophoblast cell activation and NLRP3 inflammasome activation in vitro. These findings suggested that diannexin inhibited MP release and might be an effective therapeutic strategy for preventing PE.

Keywords： preeclampsia recombinant protein diannexin microparticle NLRP3 inflammasome phosphatidylserin

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Bacteria-mediated tumor-targeted delivery of tumstatin (54-132) significantly suppresses tumor growth in mousemodel by inhibiting angiogenesis and promoting apoptosis

Frontiers of Medicine 2022, Volume 16, Issue 6, Pages 873-882 doi: 10.1007/s11684-022-0925-2

Abstract: Treatment with VNP-Tum5 effectively suppressed tumor growth and prolonged survival in the mouse modelVNP-Tum5 significantly inhibited the proliferation and migration of mouse umbilical vascular endothelial

Keywords： Salmonella VNP20009 tumstatin B16F10 melanoma apoptosis angiogenesis

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Chemotactic effect of urokinase-type plasminogen activator on mouse spermatozoa

DING Xiaofang, LI Honggang, XIONG Chengliang

Frontiers of Medicine 2008, Volume 2, Issue 2, Pages 195-199 doi: 10.1007/s11684-008-0037-7

Abstract: this study is to investigate the chemotactic effect of urokinase-type plasminogen activator (uPA) on mouseFirstly, the chemotactic effect of an ascending gradient of uPA on mouse spermatozoa was observed byMeanwhile, to confirm the specific effect of uPA on mouse sperm chemotaxis, uPA inhibitor (PAI-1) and

Keywords： chemotactic activity receptor uPA inhibitor F10 chemoattractant

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Postnatal feeding with high-fat diet induces obesity and precocious puberty in C57BL/6J mouse pups: anovel model of obesity and puberty

Rahim Ullah, Yan Su, Yi Shen, Chunlu Li, Xiaoqin Xu, Jianwei Zhang, Ke Huang, Naveed Rauf, Yang He, Jingjing Cheng, Huaping Qin, Yu-Dong Zhou, Junfen Fu

Frontiers of Medicine 2017, Volume 11, Issue 2, Pages 266-276 doi: 10.1007/s11684-017-0530-y

Abstract: The lack of a suitable animal model, however, remains a considerable challenge in obesity studies.We also developed a novel model of C57BL/6J mouse pups with HFD-induced postnatal obesity.Our model suggests good construction validity of obesity and precocious puberty.Furthermore, our model can also be used to explore the mutual interactions between diet–induced

Keywords： postnatal HFD feeding obesity kisspeptin HPG axis precocious puberty

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Novel lysosome-targeted anticancer fluorescent agents used in zebrafish and nude mouse tumour imaging

Frontiers of Chemical Science and Engineering 2022, Volume 16, Issue 1, Pages 112-120 doi: 10.1007/s11705-021-2075-5

Abstract: The design of three novel fatty nitrogen mustard-based anticancer agents with fluorophores incorporated into the alkene structure (CXL 118, CXL121, and CXL122) is described in this report. The results indicated that these compounds are selectively located in lysosomes and exhibit effective antitumour activity. Notably, these compounds can directly serve as both reporting and imaging agents in vitro and in vivo without the need to add other fluorescent tagging agents.

Keywords： fluorescent drug lysosomal anticancer zebrafish nude-mouse tumour imaging

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R158Q and G212S, novel pathogenic compound heterozygous variants in of Gitelman syndrome

Frontiers of Medicine 2022, Volume 16, Issue 6, Pages 932-945 doi: 10.1007/s11684-022-0963-9

Abstract: Meanwhile, we conducted a compound heterozygous model—Ncc R156Q/G210S mice corresponding to human NCCsup>2+ with a normal blood pressure level, which made Ncc^R156Q/G210S mice an optimal mousemodel for further study of GS.

Keywords： Gitelman syndrome mouse model compound heterozygous hypokalemia Slc12a3

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MiRNA-451 is a potential biomarker for estrogenicity in mouse uterus

Lingyan HOU, Yun LU, Ying LI, Li LI

Frontiers of Environmental Science & Engineering 2014, Volume 8, Issue 1, Pages 99-105 doi: 10.1007/s11783-013-0490-7

Abstract: test the 17β-estradiol (E2) induced miRNA expression profile at different time points in the immature mouse

Keywords： estrogen microRNA (miRNA) microarray biomarker

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Antitumor immunity of human SART3 gene vaccine against mouse tumor

HE Yu, YANG Shuhua, LIU Yong, LI Tao

Frontiers of Medicine 2008, Volume 2, Issue 1, Pages 51-57 doi: 10.1007/s11684-008-0010-5

Abstract: SART3) gene can induce antitumor immunity against tumor cells which express the gene, we constructed mouseIt was found that human SART3 DNA vaccine can elicit a specific CTL reaction from the mouse splenocytes

Keywords： antitumor therapy occurrence implantation DNA vaccine SART3 DNA

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Molecular dynamic simulation on the conformation of mouse muscle type nAChR

Shengai SUN, Rilei YU, Yanhui ZHANG, Yanni LI,

Frontiers of Chemical Science and Engineering 2010, Volume 4, Issue 3, Pages 348-352 doi: 10.1007/s11705-009-0284-4

Abstract: A mouse muscle type nAChR model ((α1)βδγ) was built based on the cryoelectron microscopicThe conformation of the pentameric nAChR model was investigated by molecular dynamic simulation.

Keywords： pentameric hydrophilic Tyr127 cryoelectron microscopic conformation

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Spatiotemporal expression of Ezh2 in the developing mouse cochlear sensory epithelium

Yan Chen,Wenyan Li,Wen Li,Renjie Chai,Huawei Li

Frontiers of Medicine 2016, Volume 10, Issue 3, Pages 330-335 doi: 10.1007/s11684-016-0459-6

Abstract: However, the detailed expression profile of Ezh2 during mouse cochlear development has not been investigated

Keywords： polycomb repressive complex Ezh2 expression inner ear cochlea development

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AML1-ETO driven acute leukemia: insights into pathogenesis and potential therapeutic approaches

Megan A. Hatlen, Lan Wang, Stephen D. Nimer

Frontiers of Medicine 2012, Volume 6, Issue 3, Pages 248-262 doi: 10.1007/s11684-012-0206-6

Abstract: Both human and mouse models of AML have demonstrated that AML1-ETO is insufficient for leukemogenesis

Keywords： AML1-ETO mouse model leukemia t(8 21) pathway hits mutation hematopoiesis Kasumi-1 CD34+

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Construction of lentiviral vector carrying Rab9 gene and its expression in mouse brain

Youguo HAO, Min ZHANG, Jinzhi XU, Bitao BU, Jiajun WEI

Frontiers of Medicine 2009, Volume 3, Issue 2, Pages 141-147 doi: 10.1007/s11684-009-0041-6

Abstract: Rab proteins and their effectors facilitate vesicular transport by tethering donor vesicles to their respective target membranes. Rab9 mediates late endosome-to- -Golgi-network trafficking. To explore the possibility of Rab9-related gene therapy for neurodegenerative diseases, we packed Lentivirus encoding Rab9. The expressing plasmid pCDH1-MCF1-Rab9-EF1-copGFP was constructed by using molecular biological techniques. The Lentivirus encoding Rab9 cDNA was packed by Lifectamine-2000 mediated co-transfection of the plasmid pPACKH1- , pPACKH1- and pVSV- into 293T cells. DNA sequencing proved the successful construction of pCDH1-MCF1-Rab9-EF1-copGFP. After 72 hours, the expression of GFP could be detected in BV-2 cells. Western blotting revealed that the Rab9 gene expression in BALB/c mice brain was up-regulated significantly 4 weeks after injection with Lentivirus encoding Rab9, which evidenced a satisfactory increasing effect of this virus. Administration of Lenti-Rab9 to postnatal day 3 Niemann-Pick disease type C (NPC) mice reduced motor defects and prevented the weight loss associated with female NPC mice, as well as modulating the death rate of Purkinje neurons. It is concluded that the packaging of Lentivirus encoding Rab9 was successful. Lentivirus encoding Rab9 can increase the expression of Rab9 gene effectively, which might offer a novel means for the treatment of neurodegenerative diseases.

Keywords： Rab9 lentivirus gene therapy gene transfer

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Keratin 5-Cre-driven deletion of

Jun Yang, Lianqing Wang, Yingzhi Huang, Keqiang Liu, Chaoxia Lu, Nuo Si, Rongrong Wang, Yaping Liu, Xue Zhang

Frontiers of Medicine 2020, Volume 14, Issue 3, Pages 305-317 doi: 10.1007/s11684-019-0722-8

Abstract: Familial acne inversa (AI) is an autoinflammatory disorder that affects hair follicles and is caused by loss-of-function mutations in -secretase component genes. We and other researchers showed that ( ) is the most frequently mutated gene in familial AI. In this study, we generated a keratin 5-Cre-driven epidermis-specific conditional knockout mutant in mice. We determined that this mutant recapitulated the major phenotypes of AI, including hyperkeratosis of hair follicles and inflammation. In mice, the IL-36a expression level markedly increased starting from postnatal day 0 (P0), and this increase occurred much earlier than those of TNF- , IL-23A, IL-1 and TLR4. RNA-Seq analysis indicated that Sprr2d, a member of the small proline-rich protein 2 family, in the skin tissues of the mice was also upregulated on P0. Quantitative reverse-transcription polymerase chain reaction showed that other genes had a similar expression pattern. Our findings suggested that IL-36a might be a key inflammatory cytokine in the pathophysiology of AI and involved in the malfunction of the skin barrier in the pathogenesis of AI.

Keywords： acne inversa mouse model interleukin 1 family member 6 small proline rich protein 2D key inflammatory

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Mouse Oocyte Enucleation with Surface Tension Assisted Method

Meng Qinggang,Zhu Shien,Zeng Shenming,Zhang Zhongcheng

Strategic Study of CAE 2001, Volume 3, Issue 11, Pages 65-69

Abstract:

In the present experiment a new simple method-Surface Tension Aided (STA)- for mouse oocyte enucleationSome of the cytoplasts produced by STA were used for mouse ear fibroblast cell nuclear transfer by electrofusion

Keywords： surface tension assisted enucleation oocyte mouse

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Title Author Date Type Operation

Lack of CFAP54 causes primary ciliary dyskinesia in a mouse model and human patients

Journal Article

Recombinant protein diannexin prevents preeclampsia-like symptoms in a pregnant mouse model via reducing

Journal Article

Bacteria-mediated tumor-targeted delivery of tumstatin (54-132) significantly suppresses tumor growth in mousemodel by inhibiting angiogenesis and promoting apoptosis

Journal Article

Chemotactic effect of urokinase-type plasminogen activator on mouse spermatozoa

DING Xiaofang, LI Honggang, XIONG Chengliang

Journal Article

Postnatal feeding with high-fat diet induces obesity and precocious puberty in C57BL/6J mouse pups: anovel model of obesity and puberty

Rahim Ullah, Yan Su, Yi Shen, Chunlu Li, Xiaoqin Xu, Jianwei Zhang, Ke Huang, Naveed Rauf, Yang He, Jingjing Cheng, Huaping Qin, Yu-Dong Zhou, Junfen Fu

Journal Article

Novel lysosome-targeted anticancer fluorescent agents used in zebrafish and nude mouse tumour imaging

Journal Article

R158Q and G212S, novel pathogenic compound heterozygous variants in of Gitelman syndrome

Journal Article

MiRNA-451 is a potential biomarker for estrogenicity in mouse uterus

Lingyan HOU, Yun LU, Ying LI, Li LI

Journal Article

Antitumor immunity of human SART3 gene vaccine against mouse tumor

HE Yu, YANG Shuhua, LIU Yong, LI Tao

Journal Article

Molecular dynamic simulation on the conformation of mouse muscle type nAChR